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A Phred quality score is a measure of the quality of the identification of the nucleobases generated by automated DNA sequencing.〔Ewing B, Hillier L, Wendl MC, Green P. (1998): Base-calling of automated sequencer traces using phred. I. Accuracy assessment. Genome Res. 8(3):175–185. PMID 9521921 (full article )〕〔Ewing B, Green P. (1998): Base-calling of automated sequencer traces using phred. II. Error probabilities. Genome Res. 8(3):186–194. PMID 9521922 (fulll article )〕 It was originally developed for Phred base calling to help in the automation of DNA sequencing in the Human Genome Project. Phred quality scores are assigned to each nucleotide base call in automated sequencer traces. Phred quality scores have become widely accepted to characterize the quality of DNA sequences, and can be used to compare the efficacy of different sequencing methods. Perhaps the most important use of Phred quality scores is the automatic determination of accurate, quality-based consensus sequences. == Definition == Phred quality scores are defined as a property which is logarithmically related to the base-calling error probabilities .〔 or For example, if Phred assigns a quality score of 30 to a base, the chances that this base is called incorrectly are 1 in 1000. The phred quality score is the negative ratio of the error probability to the reference level of expressed in Decibel (dB). 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「Phred quality score」の詳細全文を読む スポンサード リンク
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